This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chimeric RNA: Methods and Protocols is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome.
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Salva Publishing (23) Ama Fleud-Floyd (12) CloudCorner Press (11) Inc. Barcharts (8) Alex Publisher Press (7) Don Johnson (7) Wilson Campbell (7) Abul K. Abbas MBBS (6) Audible Studios (6) Quotebook Publishing (6) Apearlly Publisher (5) Atul Gawande (5) Book lovers Publishing (5) M.D. Le, Tao (5) American Medical Association (4) Arthur Zensoku (4) Bookbia Publishing (4) Emma Olivia Family Quotes (4) M.D. Bhushan, Vikas (4) メディカ出版 (4)最適なファイルサイズ
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